HuTech - a fast and intelligent antibody humanization solution

HuTech - a fast and intelligent antibody humanization solution

The traditional homology modeling technology is used to humanize the antibody, mainly based on the similarity of the primary structure of the protein and the homology simulation based on the existing protein crystal structure. Most of the existing homology modeling techniques do not analyze the existing antibody sequence data, and there are uncertainties in the mutation site during the humanization process, and the antibody characteristics such as expression level and key amino acids are Late modification predictions, etc. cannot be predicted in advance.

Humanization of murine antibodies by phage display technology requires the establishment of a humanized library of a certain capacity for panning. The process of library construction and panning takes a long time (about 2 months), and at the same time, due to the need for more The selection, sequencing, construction and affinity detection of the panned clones resulted in a waste of manpower.

Shanghai Qiwen Biotechnology Co., Ltd. is a professional service provider in the field of domestic monoclonal antibody research and development, providing a range of services from instrumentation and reagents to bioinformatics analysis. Our unique HuTech antibody humanization technology enables homology modeling of murine antibodies based on reported antibody structures in protein databases, using bioinformatics and structural biology principles to search and select humanized antibody templates. According to the environment of the original amino acid, the core amino acids of the CDR structure of the antibody are analyzed by rapid scanning, and the key amino acids are accurately replaced by the mutation, and the affinity of the antibody is ensured to the greatest extent during the humanization process. .

HuTech monoclonal antibody humanization solutions can greatly accelerate the early development of your antibodies compared to traditional phage display technology. As shown in the figure below, HuTech technology is fully optimized in terms of completion time, cost and results evaluation.

HuTech antibody humanization technology can meet customer demand for humanization of antibodies within 7 working days (starting with the time to receive the murine antibody variable region sequence). According to the software design algorithm, HuTech antibody humanization technology will rationally select humanized antibody template to minimize the HAMA (humananti-mouse antibody) reaction, and at the same time ensure the antibody biology based on the big data analysis results of the reported antibody sequences. Humanization of murine antibodies is rapidly and perfectly achieved with activity and affinity. In addition, HuTech antibody humanization technology has analyzed and predicted all humanized antibody templates in the early stage of development. The “template selection algorithm” set in the software ensures that the optimal selection will be made in the process of humanization. Humanized templates, humanized antibodies have a further improvement in expression and stability over wild-type antibodies.

Compared to humanization based on phage library construction technology, HuTech humanization technology can save you a lot of time and labor costs while maintaining the original antibody affinity. From previous success stories, HuTech humanization technology is based on the selection of human antibody templates using a localized database and a preferred human antibody template approach compared to traditional homology modeling humanization protocols. The optimization of homology modeling methods, the analysis of key amino acid sites and the analysis of the environment of amino acid residues, etc., all have unique algorithms and post-human corrections, which make the technology more rapid and maintain the original antibody affinity. Accurate humanization of antibodies.

HuTech Humanized Service Process and Duration:

HuTech Humanization Service Results:

1. Provide software and manual selection of amino acid sequences after transformation. For most projects (more than 80%), ensure at least one humanized antibody with comparable affinity to the parental antibody; for a few projects, due to the specificity of the variable region sequence, the affinity will decrease to varying degrees, we Guaranteed that the drop factor is within 2 times.

2. Provide complete antibody humanization reports, including translational modification predictions and immunogenicity predictions.

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