Human layer mucin (LN) chemiluminescence immunoassay kit basic principle and operation steps

product description

  English name   Human LN (Laminin) CLIA Kit
  Chinese name    Human laminin (LN) chemiluminescence immunoassay kit  
  Item number   E-CL-H0122c   species   Human / person
  specification   96T/Kit (8*12 strips) 48T/Kit (8*6 strips)
  Detection method    Double antibody sandwich method  
  examination range   0.156~10ng/mL   Sensitivity   0.094ng/mL

Fundamental

This kit uses a double antibody sandwich method. The anti-Human LN antibody was coated on the plate, and the LN in the specimen or standard was bound to the coated antibody during the experiment, and the free component was washed away. Biotinylated anti-Human LN antibody and horseradish peroxidase-labeled avidin were sequentially added. The anti-Human LN antibody binds to Human LN bound to the coated antibody, and biotin binds to avidin to form an immune complex, and the free component is washed away. The luminescent substrate mixture was added, the luminescent substrate was fluoresced under the catalysis of horseradish peroxidase, and the chemiluminescence value (CL) was measured by a chemiluminescence immunoassay analyzer. The LN concentration and the chemiluminescence value were positively correlated. The standard curve is used to determine the concentration of LN in the specimen.

Steps

1. Add 100 μL of standard or sample to each well and incubate at 37 ° C for 90 minutes.

    2. Pour the liquid in the well, pat dry, add 100 μL of biotinylated antibody working solution, incubate at 37 ° C for 60 minutes.

    3. Wash 3 times

    4. Add 100 μL of enzyme conjugate working solution and incubate at 37 ° C for 30 minutes.

    5. Wash 5 times

    6. Add 100 μL of substrate solution and incubate for 5 minutes at 37 °C

    7. Readings

    8. Calculation of results

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