It takes only 3 days to produce a batch of strains with a liquid strain culture incubator, which is 1/10 of the period of using the solid strain. The cost of each tank strain is 195 yuan, and it can be connected to 1733 cm of the cultivation bag 4000 bags - 5000 bags, the cost is only 0.04 yuan, is the use of solid bacteria species of 1/32. Due to the fluidity of the liquid strains, the inserted bacteria can be dispersed and germinated at different sites. About 6 hours after inoculation, the bacteria germinated and turned white. The growth of the strains could be eaten within 24 hours, so that the mixed bacteria did not spread. In the condition of 25°C, the bag can be grown for 18 days to 20 days, and the growth period is 50% using the solid strain. Formula: Pleurotus eryngii needs abundant carbon sources and nitrogen sources, especially the richer the nitrogen source, the better the mycelium growth and the higher the yield. The formula is: wood chips 65%, cotton seed shells 10%, wheat bran 23%, gypsum 1%, lime 1%. Moisture: In the growing stage of mycelium cultivation of Pleurotus eryngii, the relative humidity of air is required to be about 60%, and the relative humidity in the formation and development stage of fruit bodies is 85%-90%. During fruit body development and growth stages, water management is extremely important. The relative humidity of the mushroom house should be maintained at about 90% in the early stage, and when the fruit body is developed and near harvest, the humidity can be controlled at about 85%, which is beneficial to extend the shelf life of the fruit body. Do not spray water on the mushrooms, especially when the temperature rises, it is easy to make fruiting bodies yellow, and when it is severely punished, it will also infect the bacteria, causing rot, affecting the yield and quality. Temperature: The optimum temperature for the growth of mycelium of Pleurotus eryngii is about 25°C, and the optimum temperature for forming the primordia is 10-15°C. The developmental temperature of fruiting bodies varies with different strains, and the general temperature is 15-21°C. Light: The growth of Pleurotus eryngii does not require light, and the formation and development of fruiting bodies requires scattered light. Ventilation: The growth of fresh mycelia of Pleurotus eryngii and the development of its fruit body need fresh air, but during the growth phase of mycelium, the carbon dioxide accumulated in the bottle (bag) promotes the growth of mycelium. With the growth of mycelium, the carbon dioxide emitted by the mycelium in the culture material gradually increases, so that the concentration of carbon dioxide in the culture material rises, and the growth of the mycelium can be significantly stimulated. On rainy days, the fruiting body growth phase also requires increased ventilation. When the temperature rises above 18°C, ventilation must be increased while increasing the spray water temperature to avoid high temperature and high humidity, resulting in fruiting body rot. Production of liquid bacterium strains of Pleurotus eryngii has a biological efficiency of 80%-100%, and production of first-fruit tidal mushroom can be increased by 17%, and total production can be increased by 4.25%. Concentrated production of mushrooms can facilitate market sales. In addition, the application of liquid strains will shorten the cultivation period, thus increasing the cultivation batch and improving the utilization rate of the mushroom house. The benefit is the use of solid strains that cannot be compared. China Agricultural Network Editor