How to set the centrifugation time

Separation of serum or plasma from contact with blood cells and clots should be completed as soon as possible after the blood collection (within 2 hours): (1) Centrifugal time and relative centrifugal force (RCF): Clinical chemical analysis When the blood specimen is centrifuged, RCF (1000 to 1200) ×g, the centrifugation time is 5 to 10 min.

(2) Temperature control Centrifugation: Heat generation during centrifugation is not conducive to the stability of the analyte. Clinical chemical analysis must be performed with a temperature-controlled centrifuge when the blood specimen is centrifuged. Some temperature-dependent analytes (such as adrenocorticotropic hormone, cyclic adenosine monophosphate, catecholamines, etc.) should be separated at 4 ° C; no unusual temperature requirements of the analyte, the centrifugation temperature should be set at 20 ° C ~ 22 ° C; temperature is lower than The blood potassium measurement can be artificially increased at 15 ° C; the refrigerated specimen must be centrifuged at the required temperature.
(3) Storage of separated serum or plasma: laboratory temperature and storage temperature and time of serum or plasma are important parameters for analyte stability and accuracy of measurement results:
1 at 22 ° C ~ 25 ° C, serum or plasma preservation does not exceed 8h;
2 When the experiment cannot be completed within 8 hours, the serum or plasma should be stored at 2 ° C ~ 8 ° C;
The experimental items that cannot be completed within 348 hours, or the separated serum or plasma should be stored at -20 °C when stored for more than 48 hours;
4 specimens can not be frozen and thawed (can only freeze and thaw once), and can not be stored in a frost-free refrigerator (can cause sample temperature changes);
5 After centrifugation, the serum on the gel (gel barrier) can be stored for 2 to 5 days (also reported at 4 ° C for 24 hours), but the integrity of the gel must be ensured; The serum or plasma must be removed immediately afterwards.

6 Serum or plasma must be stored in a closed test tube.

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