Molecular diagnosis is based on nucleic acid as a test object, which is mainly used in the diagnosis of various clinical subjects, such as tumor, infection, genetics, etc., and nucleic acid extraction is the "first step" in molecular diagnostic experiments. Through the coating of magnetic beads (such as silicon, amino, carboxyl, etc.), high-throughput, automated extraction of nucleic acids can be achieved. This technology was developed in the 1980s and has mature kits. And formed into an industry.
The use of magnetic beads: in vitro diagnostics (In-Vitro Diagnostics)
When it comes to magnetic beads, it is necessary to mention the in vitro diagnostic research of the current hot, magnetic beads play an irreplaceable role in it. In vitro diagnosis (referred to as IVD) can be divided into hematological diagnosis, biochemical diagnosis, immunodiagnosis, molecular diagnosis, microbial diagnosis, urine diagnosis, coagulation diagnosis, etc. according to the different principles and methods of testing. In China, the biochemical diagnosis market is relatively mature. In immunodiagnosis, chemiluminescence and molecular diagnosis are the market segments with faster development.
According to statistics, the global IVD market has reached 55.4 billion US dollars in 2013, and is expected to reach 80 billion US dollars in 2018. Developed countries such as Europe, America and Japan are the main markets for IVD consumption. Specific to product classification, POCT, microbiology and immunodiagnosis account for a large proportion. POCT is mainly due to the dietary structure and institutional reasons of developed countries such as Europe and the United States. In the IVD market, there are many applications for rapid blood glucose testing, so it occupies a large proportion.
Principle and steps of magnetic nucleic acid extraction
Figure 1 Schematic diagram of the process of nucleic acid extraction by magnetic beads
Comparison between traditional nucleic acid extraction and magnetic beads
With the rapid development of technologies such as genetic testing, personalized drug delivery, and prenatal diagnosis, all fields of the biosphere are pursuing automation and high throughput. In this context, magnetic beads extraction of DNA is more traditional than traditional methods (Chelex100 method). The organic method, the silica method, the salting out method, etc.) are simpler, faster, safer and more environmentally friendly, and the purity and concentration of the product obtained by the specific combination of the magnetic beads and the nucleic acid are higher.
Up to now, 45 kits of more than 30 companies have been approved by the CFDA for clinical testing applications. A total of 22 companies' nucleic acid extractors have been approved by the CFDA for import and domestic production, accounting for 50%.
Table 1. Comparison of basic information between magnetic beads extraction nucleic acid method and traditional extraction method
Works of sincerity: nano magnetic beads - MagneStar
MagneStar superparamagnetic beads are nano-micro developed magnetic beads with uniform particle size, fast magnetic reaction rate, ultra high specific surface area and biological inertness due to their protein, nucleic acid, antibody and cellular properties. It has unique advantages in separation and is an ideal choice for high-throughput biological sample preparation. It is widely used in in vitro diagnostic (IVD) chemiluminescence, nucleic acid extraction, gene sequencing fragment screening, cell separation, targeted drug carriers and other important fields. MagneStar superparamagnetic beads include: magnetic bead balls, hydroxyl magnetic beads, amino magnetic beads, carboxyl magnetic beads and Protein A magnetic beads.
Advantages of MagneStar Super Paramagnetic Beads
- Multiple types of uniform particle size: core-shell type (300nm, 500nm, 1μm), monodisperse sandwich type (2μm, 3μm, 5μm)
- Ultra-fast magnetic response, only a few seconds to a dozen seconds, more than double the speed of similar products
- Magnetic content can be flexibly regulated, non-specific adsorption is low
- Provide a variety of surface functional group coatings
- More professional customer service
MagneStar Product Attributes List
* Core-shell type (PF): magnetic Fe3O4 as core, SiO2 or high polymer as outer layer, large specific surface area, high magnetic content
*Single-dispersed sandwich type (PFI): with a monodisperse polymer as the core, the middle layer is magnetic Fe3O4, and the outermost layer is an inorganic material to reduce non-specific adsorption.
Note: We can provide magnetic beads, hydroxyl beads, carboxyl beads, amino beads, more specifications or custom, please contact your business representative.
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